The gradient function enables assay optimization and the easy determination of the optimal temperature for primer annealing which is crucial for efficient and specific target amplification. This ultimately means the highest possible reproducibility from well to well and from cycle to cycle. The uncompromising performance of the QIAquant ensures fast cycling and homogeneous heat distribution across all the wells of a plate. The sample block provides outstanding thermal properties in terms of temperature uniformity across the sample block down to ± 0.15☌ at 55☌, and has an extremely fast heating and cooling rate up to 8☌/s. The QIAquant 96 instruments are fitted with a sample block made of silver coated with gold. The QIAquant instrument is fitted with high quality heating elements with gradient function and a temperature control precision of ± 0.1☌. The QIAquant instruments do not require the use of a reference dye such as ROX and do not need regular calibration.Īccurate and homogeneous temperatures across the samples are critical for the rate and efficiency of PCR assays. This enables fast read-out times of only 6 seconds for an entire plate regardless of the number of filters.
The fiber-optic shuttle system is fitted with 8 individual light fibers in the QIAquant 96 instruments and 16 individual light fibers in the QIAquant 384 instrument. Composed of 5 different-colored long-life and high power LEDs, the light source guarantees the optimal excitation of known fluorescent dyes from blue to the near-infrared range and the emission signal is captured by a sensitive photomultiplier tube (PMT). Scanning just above the sample, the optic shuttle guarantees the homogeneous excitation of each individual sample and the reliable recording of the emitted fluorescent signals with high sensitivity and no cross-talk between wells. The QIAquant instruments use a patented Fiber-Optic Shuttle System. 1.7☌/s (depending on consumables used)ġ nmol/l FAM at 30 µl sample volume in a 96-well PCR plateġ nmol/l FAM at 30 µl sample volume in a 384-well PCR plateĩ6-well plate (single measurement, 5 colors) approximately 6 sģ84-well plate (single measurement, 5 colors) approximately 6 s 1 x 96-well microplate with optical film ġ2 x 8-well strips 0.2 ml with optical lids ĩ6 x 0.2 ml individual vessels with optical lidsġ x 384-well PCR plate with optical sealing film
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